Euphresco project: Reliable detection of pathogens in soil

Euphresco call 324 of 2019 resulted on the establishment of the project: Reliable detection of pathogens in soil.

A project with 13 partners from Europe and Mediterranean region and beyond where expertise is exchanged to contribute to the Euphresco Objective 2017-R-5.2: develop and validate high-throughput DNA extraction methods.

Within this project ClearDetections will contribute to its main goals which are:

• to validate protocols for the extraction of total nucleic acid from specific soil borne pathogens such as Phytophtora spp., Fusarium spp., Pythium spp., Rhizoctonia spp., Agrobacterium spp., Meloidogyne spp., Globodera spp.
• to validate the use of large volumes of soil (>100 g) for total nucleic acid extraction with different types of soil, and validate the suitability of the total nucleic acid to be used for molecular testing (real-time PCR or barcoding)
• to compare the sensitivity of the bioassay and of the molecular tests

By being a part of this project ClearDetections ensures a key position within Plant Health stakeholders and actively contributes to bring to the community better DNA extraction tools that will improve plant health diagnostics by molecular tools.