Can I use any Real-Time PCR machine?
Our assays use a fluorescent DNA binding dye for detection and therefore will produce good results with any Real-Time PCR machine that has a FAM/SYBR channel for detection.
The wells in the PCR plate should have at least a volume of 25 uL. All our assays have a total volume of 20 uL per well (15 uL PCR mix + 5 uL DNA sample).
The ClearDetections PCR mixes do not contain a passive reference dye. If applicable, you have to deactivate any passive reference dye option in your Real-Time PCR software. Feel free to contact us for advice and support if this situation applies to you.